United States Patent:  6,083,512

Appl. No.:  536970

Novel multicomponent clostridial vaccine formulations using readily dispersible, non-depot adjuvants, such as saponin, are disclosed. The vaccines can be administered to cattle intramusculary or subcutaneously without the severe persistent local reactions, such as granulomas, abscesses, and scarring, normally seen with other multicomponent clostridial vaccines.

DISCLOSURE OF THE INVENTION

The present invention is based on the surprising discovery that the water-soluble adjuvant, saponin, can be used in place of a depot adjuvant in multicomponent clostridial vaccines for cattle. The vaccines are safe and nontoxic.

Accordingly, in one embodiment, the invention is directed to a multicomponent clostridial vaccine composition comprising two or more clostridial immunogens and a dispersible, soluble adjuvant.

In another embodiment, the subject invention is directed to a multicomponent clostridial vaccine composition comprising:

(a) clostridial bacterins or toxoids derived from each of Clostridium chauvoei, Clostridium septicum, Clostridium novyi, Clostridium sordellii, Clostridium perfringens, Type C and Clostridium perfringens, Type D; and

(b) a saponin adjuvant.

In yet another embodiment, the invention is directed to a multicomponent clostridial vaccine composition comprising:

(a) clostridial bacterins or toxoids derived from each of Clostridium haemolyticum, Clostridium chauvoei, Clostridium septicum, Clostridium novyi, Clostridium sordellii, Clostridium perfringens, Type C and Clostridium perfringens, Type D; and

(b) a saponin adjuvant.

Still other embodiments of the present invention are directed to methods of preventing or treating clostridial infection in a bovine animal, and methods comprising administering effective amounts of the subject vaccine compositions to the bovine animal.

In particularly preferred embodiments, the administering is done intramuscularly or subcutaneously.

These and other embodiments of the subject invention will readily occur to those of ordinary skill in the art in view of the disclosure herein.

Claim 1 of 21 Claims

1. A multicomponent clostridial vaccine composition, consisting of immunogens from two or more species or serotypes of Clostridium, a saponin adjuvant, and a pharmaceutically acceptable carrier.

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