Title: Food for inhibiting infection and treating
gastritis, gastric and duodenal ulcers
United States Patent: 6,329,002
Inventors: Kim; Hyun Mi (#202 728-11 Pungdukchunli, Sugieup,
Yongin, Kyunggido 449-840, KR); Heo; Cheol Seong (#305 304dong Daelip
hansup apt, Chunan, Chungchungnamdo 330-160, KR); Lee; Jung Lyoul (#904
302dong Daewon apt, chowonmayeul pyungchongdong Donganku, Anyang,
Kyungkido, 431-070, KR); Kim; Hyung Soo (52303 Brenton Hills Dr., Granger,
Appl. No.: 498668
Filed: February 7, 2000
Foreign Application Priority Data: Feb 08, 1999[KR]
(99-4234); Sep 20, 1999[KR] (99-40387)
Live strains of Lactococcus sp. HY 49, Lactobacillus casei HY 2782, and
Bifidobacterium longum HY 8001 maintained in nutritious foods, such as
yogurt, imbue them with prophylactic and/or therapeutic properties. Such
foods are beneficial in the prevention and/or treatment of gastritis,
duodenal and gastric ulcers caused by infection from Helicobacter pylori
(also referred to as H. pylori). The properties of these bacteria are
boosted by the addition of egg yolk containing antibodies specific to H.
pylori antigen derived from "fractionated H. pylori".
SUMMARY OF THE INVENTION
A natural or synthetic food is supplemented with
particular strains of live bacteria which by themselves are effective
against Helicobacter pylori (also referred to as H. pylori) not only in
vitro but in vivo. Optionally and preferably, the effectiveness of such
bacteria is boosted with egg yolk containing antibodies specific to H.
pylori antigens, so that consuming the food will prevent and/or treat
gastritis, and/or gastric and duodenal ulcers.
It has been discovered that specific strains of non-toxic (to humans) live
bacteria, by themselves, when maintained as "active strains" in
comestible foods, such as yogurt and other bacteria-tolerant foods which
may contain other living organisms, have the unique ability to imbue such
foods with prophylactic and/or therapeutic properties because, it is
hypothesized, active strains produce bacteriocins which directly attack H.
pylori. In the genus of lactic acid bacteria, only the strains Lactococcus
sp. HY 49, Lactobacilhus casei HY 2782; and in the genus of
Bifidobacterium which is closely related to lactic acid bacteria, only the
strain Bifidobacterum longum HY 8001; lodged in the Korean Culture
collection, Seoul, Republic of Korea under Depository No. KFCC 10870 for
convenience these three together are referred to herein as "active
lactic acid bacteria strains" because they have been found either to
minimize the growth of, or to destroy H. pylori not only in vitro but also
in vivo in the environment of a stomach, provided each strain is used,
either individually or in combination, in an effective dosage amount.
It is therefore a general object of this invention to provide a food for
general human consumption, comprising a food stored at a temperature in
the range from about -45oC. but no more than 45oC.
and effective to inhibit and/or prevent the growth of H. pylori in a human
stomach; the food is most preferably of lactic acid origin; the food is
fortified with an active strain selected from the group consisting of
Lactococcus sp. HY 49, Lactobacillus casei HY 2782, and Bifidobactedum
longum HY 8001, preferably a combination of all three, optionally in
combination with antibodies obtained in the yolk of an egg of a hen
immunized against a pathogenic factor selected from the group consisting
of fractionated H. pylori and urease of H. pylori. "Fractionated H.
pylori" consists essentially of a pathogenic factor selected from the
group consisting of H. pylori-urease (hereafter referred to only as "urease"),
flagella of H. pylori and outer membrane of H. pylori separated from the
rest of the mass of H. pylori cells. Portions (ii) and (iii) are typically
used together (referred to as "flagella/outer membrane") to
avoid the effort of separating them, were each to be used individually.
Urease, though not an integral portion of the H. pylori cells is referred
to as a constituent of fractionated H. pylori because it is derived from,
and separated from the comminuted cells.
It is a specific object of this invention to provide an improved food
selected from the group consisting of a conventional food of lactic acid
bacterial origin and a non-conventional food, in combination with an
effective dosage amount of an active strain selected from the group
consisting of Lactococcus sp. HY 49, Lactobacillus casei HY 2782, and
Bifidobacterium longum HY 8001, said conventional food being selected from
the group consisting of yogurt, buttermilk, cream cheese and ice cream,
and said non-conventional food being a nutritional yogurt drink. The
useful concentration of active lactic acid bacteria strain(s) in the food
is in the range from about 1x108 cfu/ml to about 1x1010
cfu/ml, preferably in the range from 5x108 cfu/ml to about 5x1010
cfu/ml, and optimally about 1x109 cfu/ml in a unit serving. The
useful concentration of H. pylori-antibodies in egg yolk is in the range
from 50 mg to about 250 mg per unit serving, typically in yogurt. The egg
yolk is typically used in a fortified food in the range from about 0.05%
to about 4% by weight of the food, preferably in the range from 0.5 to 2%,
so that at 2% the food contains less than 125 mg of antibodies.
It is another general object of this invention to provide a method of
preventing and/or treating disorders associated with infection by H.
pylori, the method comprising administering to a human a nutritional food
in combination with an effective dosage amount of one or more of the
active strains identified immediately hereinabove, in plural successive
unit servings, each unit serving spaced apart from a prior one by a period
in the range from 1 hour to 3 days; and, preferably to do so in
combination with egg yolk powder containing H. pylori-antibodies derived
from one or more of the aforespecified pathogenic factors, most preferably
all three in combination, wherein the antibodies have been stabilized with
from about 5% to about 20% by weight of a water-soluble simple sugar. By a
"unit serving" we refer to a conventional individual serving for
the particular food being fortified; for example, for yogurt, a typical
unit serving is 8 fl oz (fluid ounces) or about 450 ml, though it may
range from 4 fl oz to 12 fl oz (250 ml to 950 ml). The term "simple
sugar" refers to a carbohydrate containing an .alpha.-hydroxy
aldehyde or an .alpha.-keto primary alcohol, preferably sucrose, fructose,
lactose, glucose, dextrose and the like.
It is another specific object of this invention to provide a novel
adjuvant which is more effective to potentiate an immune response to H.
pylori antigens than conventionally used Freund's adjuvant (complete or
incomplete) and which not only produces more antibodies than with
conventional adjuvants, but also produces more than 75% of the antibodies
in the yolk of a hen immunized with an antigen of fractionated H. pylori
in the novel adjuvant.
Claim 1 of 16 Claims
1. A food for general human consumption, comprising a food stored at a
temperature in the range from about -45oC. but no more than 45oC.,
and effective to inhibit and/or prevent the growth of Helicobacter pylori
in a human stomach, said food being fortified with an amount effective to
inhibit and/or prevent the growth of H. pylori, of an active strain of a
non-toxic living microorganism selected from the group consisting of
Lactococcus sp. HY 49, Lactobacillus casei HY 2782, and Bifidobacterium
longum BY 8001 said strain by itself being effective against H. pylori not
only in vitro but also in vivo and produces bacteriocins which directly
attack H. pylori.
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