Title:  Treatment of C. difficile toxin B associated conditions

United States Patent:  6,465,435

Issued:  October 15, 2002

Inventors:  Heerze; Louis D. (Edmonton, CA); Armstrong; Glen D. (Edmonton, CA)

Assignee:  SYNSORB Biotech, Inc. (Calgary, CA)

Appl. No.:  593040

Filed:  June 13, 2000

This invention relates to prevention and/or treatment of antibiotic associated diarrhea, including Clostridium difficile associated diarrhea (CDAD), pseudomembranous colitis (PMC) and other conditions associated with C. difficile infection, using oligosaccharide compositions which bind C. difficile toxin B. More specifically, the invention concerns neutralization of C. difficile toxin B associated with such conditions.

DETAILED DESCRIPTION OF THE INVENTION

A. Definitions

As used herein the following terms have the following meanings:

The term "antibiotic-associated bacterial diarrhea" refers to the condition wherein antibiotic therapy disturbs the balance of the microbial flora of the gut, allowing pathogenic organisms such as Clostridium difficile to flourish. These organisms cause diarrhea. Antibiotic-associated bacterial diarrhea includes such conditions as Clostridium difficile associated diarrhea (CDAD) and pseudomembranous colitis (PMC).

The term "biocompatible"refers to chemical inertness with respect to human tissues or body fluids.

The terms "compatible linker arm" or "linker arm" refer to a moiety which serves to space the oligosaccharide structure from the biocompatible support and which is bifunctional wherein one functional group is capable of binding to a reciprocal functional group of the support and the other functional group is capable of binding to a reciprocal functional group of the oligosaccharide structure. Compatible linker arms preferred in the present invention are non-peptidyl spacer arms. The oligosaccharide may be linked via an 8-methoxycarbonyloctyl linker or via another appropriate non-peptidyl linker, such as a urea-like linker arm of the formula --NH--(CH₂)_m --NHC(O)NH--, where m is an integer of from about 2 to about 10.

The term "oligosaccharide" means saccharides comprising 1 to about 20 saccharide moieties. Saccharide derivatives may also be used as saccharide moieties included in the term oligosaccharide [67-69].

The term "pseudomembranous colitis" (PMC), also known as pseudomembranous enterocolitis or enteritis, refers to the inflammation of the mucous membrane of both small and large intestine with the formation and passage of pseudomembranous material (composed of fibrin, mucous, necrotic epithelial cells and leukocytes) in the stools.

The term "support" refers to an inert material to which the oligosaccharide sequences may be bound or immobilized via a compatible linker arm. Where use is in vivo, the support will be biocompatible.

The term "SYNSORB" refers to 8-methoxycarbonyloctyl oligosaccharide structures covalently coupled to Chromosorb P.TM. (Manville Corp., Denver, Colo.) [12], a derivatized silica particle material. Where indicated, the SYNSORB may use a urea-like linker arm rather than the 8-methoxycarbonyloctyl linker.

The term "toxin A" refers to an enterotoxin of Clostridium difficile which initiates CDAD and related conditions. This toxin has a lectin-like activity.

The term "toxin B" refers to a cytotoxin of Clostridium difficile which causes destruction of intestinal cells and induces the release of inflammatory mediators.

For purpose of this application, all sugars are referenced using conventional three letter nomenclature. All sugars are assumed to be in the D-form unless otherwise noted, except for fucose, which is in the L-form. Further all sugars are in the pyranose form.

B. Pharmacology Amino acid sequences in C. difficile toxin A and B that are similar to sequences responsible for oligosaccharide binding in Streptococcal glucan binding proteins have been reported [49-51]. Although, as noted above, the receptor for toxin B is not known, the oligosaccharide binding specificity for these glucan binding proteins is for repeated glucose units linked together as shown below [52]:

.alpha.Glc(1.fwdarw.6).alpha.Glc(1.fwdarw.6).alpha.Glc . . .

The oligosaccharide isomaltotriose (.alpha.Glc(1-6).alpha.Glc(1-6)Glc) was immobilized by attachment onto Chromosorb P using a linker arm, and tested in toxin B neutralization experiments. The results from these experiments are presented graphically in FIGS. 1A and 1B, where concentration and time dependent neutralization of C. difficile toxin B cytotoxic activity using immobilized isomaltotriose SYNSORB (n=3) is shown. Concentration neutralization experiments were performed by incubating immobilized isomaltotriose (10, 20 or 40 mg) with 1 mL of toxin B for 2 hours at room temperature. The amount of toxin activity in each sample was measured using Chinese hamster ovary (CHO) cells.

The results are presented as the percent activity remaining relative to control toxin solutions that had not been incubated with SYNSORB. Time dependent neutralization experiments were performed by incubating toxin B with 20 mg samples of immobilized isomaltotriose SYNSORB for 1, 2 and 4 h at room temperature. A control incubation (4 h) of toxin B with Chromosorb P was included to determine the extent of background binding to the support. The results are presented as the percent activity remaining relative to control toxin solutions that had not been incubated with SYNSORB and indicate that toxin B bound to isomaltotriose SYNSORB in a concentration and time dependent manner. The results also indicated that toxin B binds to the support slowly, requiring up to 4 hours to achieve significant toxin B binding under these conditions. Further, these data show that oligosaccharides which contain .alpha.(1-6)-linked repeating units of glucose are effective at binding toxin B and can serve as a therapeutic for C. difficile-mediated diarrhea.

SYNSORBs which incorporate oligosaccharides terminating in glucose or N-acetylglucosamine were also examined for toxin B binding by measuring the cytotoxic activity of toxin B with or without SYNSORB in CHO cells. Results of these studies are shown in Table 1, where * indicates SYNSORBs using the urea-like linker arm.

 

All SYNSORBS tested except SYNSORB 23 effectively neutralized toxin B cytotoxicity. By comparison, toxin A did not bind to the SYNSORBs 5-128 (isomaltotriose) and 179A (isomaltose). The other SYNSORBs in Table 1 were not tested against toxin A. This observation confirms that there are differences in the binding specificity of toxin A and toxin B, even though there is some amino acid homology (60% amino acid homology) between the two toxins. Oligosaccharides which bind toxin A have been identified [65-66].

We also utilized a SYNSORB derivative that incorporates two different oligosaccharide ligands. The ligands selected for the dual labelling of Chromosorb P.TM. were based on previous results which revealed differential oligosaccharde binding specificities for toxins A and B. Since the oligosaccharide .alpha.Gal(1-3).beta.Gal(1-4).beta.Glc (Cd) binds toxin A but not toxin B, it was selected for use as the toxin A neutralizing component, and was immobilized onto amino derivatized Chromosorb P using an 8-methoxycarbonyl octyl linker arm. Toxin B but not toxin A binds to isomaltose (.alpha.Glc(1-6)Glc). Utilizing the amino derivatized Chromosorb that already incorporated the Cd oligosaccharide, isomaltose was immobilized onto the support using the recently developed "Instasorb" linker arm technology as disclosed in PCT/CA97/00851 [70]. The resulting SYNSORB (SYNSORB 5174, which has both oligosaccharides covalently bound by their respective linkers) was then tested for toxin A and B binding. SYNSORB Cd and isomaltose SYNSORB (SYNSORB 179A) were included as controls. The results, presented in FIG. 3, show that SYNSORB 5174 neutralized both toxin A and B activity. The results also indicate the toxin neutralizing capacity of SYNSORB 5174 was comparable to SYNSORB Cd and SYNSORB 179A. Thus, a support comprising more than one oligosaccharide ligand can be used to bind both toxin A and toxin B.

C. Synthesis

Chemical methods for the synthesis of oligosaccharide structures can be accomplished by methods known in the art. These materials are generally assembled using suitably protected individual monosaccharides.

The specific methods employed are generally adapted and optimized for each individual structure to be synthesized. In general, the chemical synthesis of all or part of the oligosaccharide glycosides first involves formation of a glycosidic linkage on the anomeric carbon atom of the reducing sugar or monosaccharide. Specifically, an appropriately protected form of a naturally occurring or of a chemically modified saccharide structure (the glycosyl donor) is selectively modified at the anomeric center of the reducing unit so as to introduce a leaving group comprising halides, trichloroacetimidate, acetyl, thioglycoside, etc. The donor is then reacted under catalytic conditions well known in the art with an aglycon or an appropriate form of a carbohydrate acceptor which possesses one free hydroxyl group at the position where the glycosidic linkage is to be established.

A large variety of aglycon moieties are known in the art and can be attached with the proper configuration to the anomeric center of the reducing unit. Appropriate use of compatible blocking groups, well known in the art of carbohydrate synthesis, will allow selective modification of the synthesized structures or the further attachment of additional sugar units or sugar blocks to the acceptor structures.

After formation of the glycosidic linkage, the saccharide glycoside can be used to effect coupling of additional saccharide unit(s) or chemically modified at selected positions or, after conventional deprotection, used in an enzymatic synthesis. In general, chemical coupling of a naturally occurring or chemically modified saccharide unit to the saccharide glycoside is accomplished by employing established chemistry well documented in the literature [21-37].

The supports to which the oligosaccharide structures of the present invention are bound or immobilized include a wide variety of biocompatible materials known in the art. Water soluble biocompatible polymers such as hydrogels, carboxymethyl celluloses, synthetic polymers, and the like are particularly preferred. In particular, these supports are useful for delivery to the gut, especially prolonged delivery. Useful supports are non-absorbable, that is to say that they may be soluble or insoluble, so long as they are not absorbed by the body.

Solid supports are particularly useful for certain applications. Such solid supports to which the oligosaccharide structures of the present invention are bound may be in the form of sheets or particles. A large variety of biocompatible solid support materials are known in the art. Examples thereof are silica, synthetic silicates such as porous glass, biogenic silicates such as diatomaceous earth, silicate-containing minerals such as kaolinite, and synthetic polymers such as polystyrene, polypropylene, and polysaccharides. Preferably the solid supports have a particle size of from about 10 to 500 microns for in vivo use. In particular, particle sizes of 100 to 200 microns are preferred.

The oligosaccharide structure(s) is covalently bound or noncovalently (passively) adsorbed onto the support so as to be immobilized. The covalent bonding may be via reaction between functional groups on the support and the compatible linker arm of the oligosaccharide structure. It has unexpectedly been found that attachment of the oligosaccharide structure to the biocompatible support through a compatible linking arm provides a product which, notwithstanding the support, effectively removes toxin. Linking moieties that are used in indirect bonding are preferably organic bifunctional molecules of appropriate length (at least one carbon atom) which serve simply to distance the oligosaccharide structure from the surface of the support.

The compositions of this invention are preferably represented by the formula:

(OLIGOSACCHARIDE-Y-R)_n -SUPPORT

where OLIGOSACCHARIDE represents an oligosaccharide group of at least 1 sugar unit which group binds to toxin B or toxins A and B, Y is oxygen, sulfur or nitrogen, R is an aglycon linking arm of at least 1 carbon atom, SUPPORT is as defined above, and n is an integer greater than or equal to 1. Oligosaccharide sequences containing about 2 to 10 saccharide units may be used. Sequences with about 2 to 4 saccharide units are preferred. In some instances, more than one oligosaccharide group may be linked to the support, e.g., one oligosaccharide group which binds toxin B and another which binds toxin A, to provide a composition which binds to more than one toxin moiety.

Numerous aglycon linking arms are known in the art. For example, a linking arm comprising a para-nitrophenyl group (i.e., --OC₆ H₄ pNO₂) has been disclosed [38]. At the appropriate time during synthesis, the nitro group is reduced to an amino group which can be protected as N-trifluoroacetamido. Prior to coupling to a support, the trifluoroacetamido group is removed thereby unmasking the amino group.

A linking arm containing sulfur has been disclosed [39]. Specifically, the linking arm is derived from a 2-bromoethyl group which, in a substitution reaction with thionucleophiles, has been shown to lead to linking arms possessing a variety of terminal functional groups such as, --OCH₂ CH₂ SCH₂ CO₂ CH₃ and --OCH₂ CH₂ SC₆ H₄ -pNH₂. These terminal functional groups permit reaction to complementary functional groups on the support, thereby forming a covalent linkage to the support. Such reactions are well known in the art.

A 6-trifluoroacetamido-hexyl linking arm, (--O--(CH₂)₆ --NHCOCF₃) has been disclosed [40]in which the trifluoroacetamido protecting group can be removed, unmasking the primary amino group used for coupling.

Other exemplifications of known linking arms include the 7-methoxycarbonyl-3,6,dioxaheptyl linking arm [41] (--OCH₂ --CH₂)₂ OCH₂ CO₂ CH₃); the 2-(4methoxycarbonylbutan-carboxamido) ethyl [42] (--OCH₂ CH₂ NHC(O)(CH₂)₄ CO₂ CH₃); the allyl linking arm [43] (--OCH₂ CH.dbd.CH₂) which, by radical co-polymerization with an appropriate monomer, leads to copolymers; other allyl linking arms [44] are known (--O(CH₂ CH₂ O)₂ CH₂ CH.dbd.CH₂). Additionally, allyl linking arms can be derivatized in the presence of 2-aminoethanethiol [45] to provide for a linking arm --OCH₂ CH₂ CH₂ SCH₂ CH₂ NH₂. Other suitable linking arms have also been disclosed [21-23, 25, 26]. The particular linking employed to covalently attach the oligosaccharide group to the support is not critical.

We have found that synthetic oligosaccharide sequences covalently attached to a biocompatible support, e. g., Chromosorb P.TM. (SYNSORB) may be used to bind toxin B. These compositions are useful to treat or prevent CDAD, PMC and other conditions associated with C. difficile infection. When a solid support is to be used, SYNSORB is particularly preferred for these compositions because it is non-toxic and resistant to mechanical and chemical degradation.

In studies using rats (a widely accepted model for preclinical studies, since they are predictive of human response), SYNSORBs have been found to pass unaffected through the rat gastrointestinal tract. They were found to be eliminated completely and rapidly (99%.eliminated in 72 hours) following oral administration. Additionally, the high density of oligosaccharide moieties on SYNSORBs is particularly useful for binding toxins which have carbohydrate binding affinity. For example, toxin A is thought to possess multiple oligosaccharide binding sites [11].

Non-peptidyl linking arms are preferred for use as the compatible linking arms of the present invention. The use of glycopeptides is not desirable because glycopeptides contain several, often different, oligosaccharides linked to the same protein. Glycopeptides are also difficult to obtain in large amounts and require expensive and tedious purification. Likewise, the use of BSA or HSA conjugates is not desirable due to questionable stability in the gastrointestinal tract when given orally.

Covalent attachment of an oligosaccharide group containing a toxin B binding unit through a non-peptidyl spacer arm to an inert support permits efficient binding and removal of toxin B or toxins A and B from a sample to be analyzed for the presence of toxin B (or toxins A and B) or from the intestine of a patient suffering from or susceptible to CDAD, PMC or another condition associated with C. difficile infection. When the oligosaccharide is synthesized with this compatible linker arm attached (in non-derivatized form), highly pure compositions may be achieved which can be coupled to various supports.

D. Pharmaceutical Compositions

The methods of this invention are achieved by using pharmaceutical compositions comprising one or more oligosaccharide structures which bind toxin B attached to a support.

When used for oral administration, which is preferred, these compositions may be formulated in a variety of ways. It will preferably be in liquid or semisolid form. Compositions including a liquid pharmaceutically inert carrier such as water may be considered for oral administration. Other pharmaceutically compatible liquids or semisolids, may also be used. The use of such liquids and semisolids is well known to those of skill in the art. (See, e.g., Remington's Pharmaceutical Sciences, 18th edition, 1990.)

Compositions which may be mixed with liquid or semisolid foods such as enteral nutritional formulas, applesauce, ice cream or pudding may also be preferred. Formulations, such as SYNSORBs, which do not have a disagreeable taste or aftertaste are preferred. A nasogastric tube may also be used to deliver the compositions directly into the stomach.

Solid compositions may also be used, and may optionally and conveniently be used in formulations containing a pharmaceutically inert carrier, including conventional solid carriers such as lactose, starch, dextrin or magnesium stearate, which are conveniently presented in tablet or capsule form. The (OLIGOSACCHARIDE-Y-R)_n -SUPPORT composition itself may also be used without the addition of inert pharmaceutical carriers, particularly for use in capsule form.

Doses are selected to provide neutralization and elimination of toxin B found in the gut of effected or at risk subjects. Useful doses are from about 0.25 to 1.25 micromoles of oligosaccharide/kg body weight/day, preferably about 0.5 to 1.0 micromoles of oligosaccharide/kg body weight/day. Using SYNSORB compositions, this means about 0.5 to 1.0 gram SYNSORB/kg body weight/day, which gives a concentration of SYNSORB in the gut of about 20 mg/ml. For subjects with clinical symptoms, administration is expected to be 3 or 4 times daily, for a period of one week or until clinical symptoms are resolved. For at risk subjects, prolonged prophylactic administration, e.g., in enteral nutritional formulas, is indicated. The dose level and schedule of administration may vary depending on the particular oligosaccharide structure used and such factors as the age and condition of the subject.

As discussed previously, oral administration is preferred, but formulations may also be considered for other means of administration such as per rectum. The usefulness of these formulations may depend on the particular composition used and the particular subject receiving the treatment. These formulations may contain a liquid carrier that may be oily, aqueous, emulsified or contain certain solvents suitable to the mode of administration.

Compositions may be formulated in unit dose form, or in multiple or subunit doses. For the expected doses set forth previously, orally administered liquid compositions should preferably contain about 1 micromole oligosaccharide/ml.

E. Methodology

We have found that C. difficile toxin B may be neutralized by certain oligosaccharide sequences which bind the toxin. In particular, synthetic oligosaccharides covalently attached to supports via non-peptidyl compatible linker arms have been found to neutralize toxin B or toxins A and B effectively. Examples of such compositions are certain SYNSORBs, which neutralize the activity of toxin B or toxins A and B.

We have tested the ability of several oligosaccharide sequences attached to Chromosorb P via 8-methoxylcarbonyloctyl (MCO) or urea-like spacer arms to neutralize toxin B or toxins A and B. The oligosaccharde sequences attached to supports useful in the present invention are those which bind toxin B and, in some cases, both toxins A and B.

The binding affinity of an oligosaccharide to toxin B is readily detectable by a simple in vitro test, as for example, set forth in Example 3 below. For the purposes of this invention, oligosaccharide sequences attached to supports which bind toxin B means those compositions which reduce cytotoxicity in CHO cell assays by at least 50%.

Several different oligosaccharide sequences attached to supports via compatible linker arms have been found to have the ability to neutralize toxin B (and in some cases, toxin A and B) activity. These sequences, and others that also bind toxin B, may be used to treat or prevent CDAD, PMC and other conditions associated with C. difficile infection. The optimal time for complete removal of toxin B activity was found to be about 4 hours at 37^oC., using a concentration of SYNSORB of 20 mg in 1 ml sample. Since each gram of SYNSORB contains approximately 0.25 to 1.0 micromoles oligosaccharide, the total amount of oligosaccharide to be given in a daily dose would range from 7.5 to 30 micromoles, using a gut volume of four liters.

Treatment or prevention of CDAD, PMC or other conditions associated with C. difficile infection may be accomplished by oral administration of compositions containing oligosaccharide sequences covalently bound to a support via a compatible linker arm (e.g., SYNSORBs). For example, SYNSORBs have been found to pass through the stomach of rats intact. This means that they are intact when they contact toxin B in the intestinal tract. Subsequent elimination of intact SYNSORB with toxin B bound to it results in elimination of toxin B from the patient.

Oligosaccharide sequences covalently attached via compatible linker arms to a support, e.g., SYNSORBs, are useful to treat individuals who suffer from multiple episodes of diarrhea. Upon initial reoccurrence of diarrhea, patients would be treated with SYNSORB to remove toxin B or both toxin A and toxin B from the intestine. The removal of toxin A prevents the initial tissue damage to the intestinal lining, which leads to prevention or reduction of diarrhea. Removal of toxin B prevents the cytotoxicity of this toxin to the intestinal and colonic cells, which also leads to prevention or reduction of diarrhea. No further treatment with antibiotics need be given, allowing the re-establishment of the normal intestinal microflora within the gut. The advantage of such treatment is that it does not affect the recolonization of the intestinal tract by normal microflora. Treatment until discontinuance of diarrhea would allow complete recovery.

In addition to its usefulness in patients suffering from recurring diarrhea, treatment with oligosaccharide sequences covalently attached via compatible linker arms to supports, e.g., SYNSORBs, may be used to treat all individuals who suffer from or are prone to develop CDAD, PMC or other conditions associated with C. difficile infection. The use of the oligosaccharide-support compositions of the present invention in combination with antibiotic therapy will be able to reduce the diarrhea more effectively, leading to more rapid recovery.

Toxin B and/or toxin A may be measured directly on the surface of the oligosaccharide-containing support using any suitable detection system. For example, radioactive, biotinylated or fluorescently labelled monoclonal or polyclonal antibodies specific for the toxin may be used to determine the amount of toxin bound to the support. A wide variety of protocols for detection of formation of specific binding complexes analogous to standard immunoassay techniques is well known in the art.

Claim 1 of 12 Claims

What is claimed is:

1. A pharmaceutical composition useful in treating or preventing Clostridium difficile associated diarrhea (CDAD) and related conditions initiated by C. difficile toxin B, which composition comprises:

a) at least one oligosaccharide, sequence covalently attached to a pharmaceutically acceptable inert support through a non-peptidyl compatible linker arm, wherein said oligosaccharide sequence binds C. difficile toxin B; and

b) a pharmaceutically acceptable carrier, wherein said composition is capable of being eliminated from the gastrointestinal tract.

 

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