Title:  Radiation cross-linked hydrogels

United States Patent:  6,537,569

Issued:  March 25, 2003

Inventors:  Cruise; Gregory M. (Rancho Santa Margarita, CA)

Assignee:  MicroVention, Inc. (Aliso Viejo, CA)

Appl. No.:  783762

Filed:  February 14, 2001

Radiation-crosslinked, biodrgradable, synthetic hydrogels and their use in various applications, including certain medical applications wherein the hydrogel(s) are implanted on or in the body of a human or animal patient. Radiation-crosslinked, biodrgradable, synthetic hydrogels of this invention may be prepared by irradiating monomers (e.g., ethylenically unsaturated hydrocarbons such as acrylic monomers and methacrylic monomers) or polymers, some or which are biodegradable or which contain biodegradable units or subunits. Specific medical applications of these radiation-crosslinked, biodrgradable, synthetic hydrogels include applicatins wherein the hydrogel is used for hemostasis, tissue augmentation, tissue engineering, embolization, closure of vascular punctures or wounds and other medical applications.

DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS

The following detailed description and examples are provided for the limited purpose of illustrating exemplary embodiments of the invention and not for the purpose of exhaustively describing all possible embodiments of the invention.

Set forth herebelow are examples of methods for preparing synthetic, biodegradable, radiation crosslinked hydrogels of the present invention, as well as some examples of methods for using such hydrogel in certain biomedical applications. The synthetic, biodegradable, radiation-crosslinked hydrogels of the present invention may be prepared from either polymeric or monomeric starting materials, and detailed examples of both types of processes are provided herebelow:

Synthesis of Biodegradable, Radiation-Croslinked Hydrogel from Polymeric Starting Materials

1. Preparation of a Macromeric Solution

Initially, a macromeric solution, comprised of aqueous or nearly aqueous solutions of polymers, is prepared. The macromer is comprised of two distinct regions, a water soluble region and a biodegradable region.

The macromer can be any biocompatible, water soluble polymer, including poly(ethylene glycol), poly(ethylene oxide), poly(ethylene glycol-co-propylene glycol), poly(vinyl pyrrolidinone), poly(vinyl alcohol), poly(ethyloxazoline), acrylic polymers, and methacrylic polymers. In a preferred embodiment, the macromer is poly(ethylene glycol). Poly(ethylene glycol) (PEG)is preferred due to its biocompatibility, ready availability in a variety of molecular weights, and its hydroxyl groups for covalently coupling the degradable moiety. Monofunctional macromers, such as monomethoxyPEG (mPEG), are particularly preferred. Preferred macromeric molecular weights range from about 2,000 to about 30,000, more preferably about 2,000 to about 15,000, most preferably about 2,000 to about 5,000.

The degradable moiety is incorporated into the macromer to impart biodegradation. The degradable moiety may undergo degradation by hydrolysis or by enzymatic action. The rate of degradation can be controlled by selecting the type of degradable region or moiety. For example, one method of imparting biodegradation is by joining two molecules of the macromer with linkages that are susceptible to hydrolysis. Examples of hydrolytically-degradable linkages that may be used include ester, peptide, anhydride, orthoester, phosphazine, and phosphoester bonds.

Also, The degradable regions can be comprised of polymer or oligomers of glycolide, lactide, .epsilon.-caprolactone, other hydroxyacids, and other biologically degradable polymers that yield byproducts that are non-toxic. Preferred poly(.alpha.-hydroxy acids) are poly(glycolic acid), poly(DL-lactic acid), and poly(L-lactic acid). Other potential degradable regions are comprised of poly(amino acids), poly(anhydrides), poly(orthoesters), poly(phosphazines), and poly(phosphoesters). Polylactones such as poly(.epsilon.-caprolactone), poly(.delta.-valerolactone), and poly(.gamma.-butyrolactone) are also useful.

Alternatively, enzymatically degradable regions can be used. Examples of enzymatically degradable regions that can be incorporated include the peptide sequences such as Leu-Gly-Pro-Ala (linkage susceptible to degradation by collagenase) or Gly-Pro-Lys (linkage susceptible to degradation by plasmin) and/or other enzymatically degradable peptide sequences as disclosed in West, Pratt and Hubbell, Protolytically Degradable Hydrogels, 23^rd Annual Meeting of the Society for Biomaterials (1997), the entirety of which is expressly incorporated herein by reference.

Once the macromer has been synthesized, an aqueous, or nearly aqueous, solution of the macromer is prepared. The solution can be prepared in water, saline, buffered saline (e.g. phosphate, carbonate, etc.), or mixtures of the above liquids with small amounts of a pharmaceutically acceptable solvent (e.g. dimethylsulfoxide, ethanol, etc.). Preferred concentrations of the macromer range from approximately 5% w/w to approximately 30% w/w.

Optionally, if the degradable moiety is susceptible to hydrolysis, the pH of the solution can be adjusted to reduce the rate of hydrolysis before implantation into or upon a mammalian body. The macromeric solution is then packaged in an airtight container. The container should be selected with care, as the hydrogel will conform to the shape of the container.

2. Crosslinking of the Macromers

The macromeric solution is then irradiated with ionizing radiaiton. The radiation causes the formation of free radicals at locations along the polymer chains and, at those free radical sites, the chains become crosslinked together. The source of the radiation could be an electron beam generator, gamma ray source, or a van de Graaff generator. Preferred dosages of radiation range from about 10 kGy to about 50 kGy. The exact radiation dosage is dependent upon the molecular weight and concentration of the macromeric solution as well as the desired mechanical properties of the resulting hydrogel. The mechanical properties of the hydrogel are controlled by the crosslink density. The crosslink density can best be manipulated through the macromer molecular weight, macromer concentration, and radiation dose. For most macromers, the crosslink. density can be increased with increasing molecular weight, concentration, and radiation dose. However for PEG based hydrogels, crosslink density is inversely proportional to the concentration of PEG. Optimization of these three variables will be required on an indication by indication basis.

3. Optional Drying or Lypohylization, Trituration and Storage

If desired, the hydrogel may be dried or, lyophilized, ground or broken into particles, and preferably stored in an inert atmosphere to enhance long term stability.

EXAMPLE 1

Preparation of a Biodegradable PEG Hydrogel form mPEG-SA-mPEG Macromer

A 500 mL round bottom flask is charged with 50 g mPEG 5,000 (Union Carbide) and 500 mL toluene (Aldrich).

Approximately 100 mL toluene is azeotropically distilled to remove water from the solution. The solution is cooled to approximately 10^oC. Succinic chloride (0.78 g, Aldrich) and triethylamine (1.00 g, Aldrich) are added to the solution. The reaction is then allowed to proceed for 48 hr at room temperature, resulting in precipitate formation.

The precipitate is removed by vacuum filtration with a fritted funnel. The mPEG dimer is then precipitated by adding the solution to 2 L of 4^o C. isopropyl alcohol (Aldrich). The precipitated mPEG dimer is collected by vacuum filtration with a fritted funnel. The dissolution in toluene/precipitation in isopropyl alcohol was repeated for three times. Finally, the mPEG dimer is dried in a vacuum oven at 50^oC.

The macromer solution is prepared by dissolving 17.5 g of the mPEG dimer in 82.5 g of 50 mM sodium phosphate pH 5. After dissolution is complete, the solution is degassed under vacuum. The macromer solution is then placed into 5 cc syringes (Becton-Dickinson) and capped. The syringes are irradiated with 30 kGy of electron beam radiation (Nutek, Inc., Hayward, Calif.), thereby forming the synthetic, biodegradable, radiation crosslinked hydrogel.

Applications of Hydrogels of the Present Invention

The following are examples of some of the biomedical applications of the biodegradable, radiation crosslinked hydrogel described above. It will be appreciated, however, that this hydrogel material has many other medical and non-medical applications in addition to the specific examples set forth herebelow.

Vascular Puncture Closure

For vascular puncture closure, a hydrogel with high adhesivity and high cohesiveness is desired. The hydrogel must adhere firmly to the subcutaneous tissues to seal the arteriotomy under arterial pressure. Furthermore, the hydrogel must have sufficient cohesive strength to prevent fragmentation under arterial pressure. Degradation of the hydrogel should occur within 1 month post-operative. Another preferred characteristic of the hydrogel for vascular puncture closure is extrudability, namely being able to be injected through no larger than a 6 Fr lumen. A preferred macromer for vascular puncture closure is a dimer of mPEG 10,000 with poly(glycolide) as a degradable linking group. A preferred formulation for vascular puncture closure is approximately 15% w/w solution of the macromer irradiated at approximately 25 kGy and maintained in a flowable, wet state.

Embolization

For embolization of tumors, arterio-venous fistula, sites of trauma or iatrogenic hemorage, etc., a hydrogel of the present invention having high cohesivity and low adhesivity is desired. The hydrogel preferably will remain intact for a period of time (e.g., several years) before it biodegrades and preferably will not deform under arterial pressure. The rate of degradation of the hydrogel may be selected or optimized for each particular embolization indication. In this regard, the rate of degredation of the hydrogel is dependent upon the type and number of biodegradable segments. If rapid biodegredation is desired, lactide or poly(lactide) may be used as a preferred biodegradable segment. If relatively slow biodegredation is desired,.epsilon.-caproloctone may be used as a preferred biodegradable segment. A preferred hydrogel for many embolozation applications may comprise dimers of mPEG 5,000 joined together by succinic chloride linking group groups (i.e., degradable moieties). A preferred formulation for embolization is approximately 25% w/w solution of the macromer irradiated at approximately 35 kGy, dried or lyophilized and formed into particles of approximately 100 to 900 microns in diameter.

Surgical or Hemostatic Sponge

As a surgical sponge, a hydrogel with high cohesiveness and some adhesiveness is desired. The hydrogel must remain intact and not deform under arterial pressure. A quick rate of degradation of a surgical sponge is desired. A preferred macromer for sponges is a dimer of mPEG 5,000 with succinic chloride as a degradable linking group. A preferred formulation for embolization is approximately 20% w/w solution of the macromer irradiated at approximately 30 kGy and dried in a sheet of desired thickness, for example approximately 0.5 cm thick. The sheet may be cut into individual sponges of desired sizes and/or shapes.

Surgical Sealant

As a surgical sealant, a hydrogel of the present invention having high adhesivity and high cohesivity is desired. Such surgical sealant may be used to repair leaking lung tissue, close endoleaks of endovascular grafts, seal leakage around sutures, staples, etc. In these applications, the hydrogel must adhere firmly to tissue to seal the fluid leak. Furthermore, the hydrogel must have sufficient cohesive strength to prevent fragmentation under load. Degradation of the hydrogel should occur within 1 month post-operative. A preferred macromer for a surgical sealant is a dimer of mPEG 10,000 with poly(glycolide) as a degradable linking group. A preferred formulation for a surgical sealant is approximately 15% w/w solution of the macromer irradiated at approximately 25 kGy and maintained in a wet, flowable state for application to the desired tissue surface.

Flowable Hemostatic Agent

For use as flowable hemostatic agents, hydrogels of the present invention having high adhesivity and high cohesivity is desired. The hydrogel must adhere firmly to tissue to seal the blood leak. Furthermore, the hydrogel must have sufficient cohesive strength to prevent fragmentation under load. Optionally, thrombin may be incorporated into the hydrogel to aid hemostasis. Degradation of the hydrogel should occur within 1 month post-operative. A preferred macromer for a hemostatic agent is a dimer of mPEG 10,000 with poly(glycolide) as a degradable linking group. A preferred formulation for a hemostatic agent is approximately 15% w/w solution of the macromer irradiated at approximately 25 kGy and maintained in a wet, flowable state.

Temporary Tissue Augmentation

For use in tissue augmentation applications, biodegradable hydrogels of the present invention having high cohesivity and no adhesivity are desired. The hydrogels used in these applications will preferably remain in tact for approximately 1 week to 1 year before biodegrading and preferably will not migrate from the original site of implantation. For tissue augmentation, a slow rate of degradation of is desired. A preferred macromer for tissue augmentation is a dimer of mPEG 5,000 with succinic chloride as a degradable linking group. A preferred formulation for embolization is approximately 20% w/w solution of the macromer irradiated at approximately 35 kGy. Examples of applications where such temporary tissue augmentation may be used include cosmetic lip enlargement, wrinkle reductions and other applications where collagen injections are currently used.

Tissue Engineering

For tissue engineering, a hydrogel with high cohesivity and no adhesivity is desired. The hydrogel must remain intact and not migrate. For tissue engineering, a moderate of degradation of is desired. The effect of the hydrogel matrix may be enhanced by the incorporation of gene therapy. vectors and/or matrix proteins or peptides. A preferred macromer for tissue augmentation is a dimer of mPEG 5,000 with poly(lactide) as a degradable linking group. A preferred formulation for embolization is approximately 20% w/w solution of the macromer irradiated at approximately, 35 kGy.

Drug Delivery

For drug delivery, a hydrogel with high cohesivity and moderate adhesivity is desired. The hydrogel must remain intact and remain where placed. The rate of degradation of the hydrogel would be dependent upon the particular drug being delivered. A preferred macromer for drug delivery is a dimer of mPEG 5,000 with succinic chloride as a degradable linking group. A preferred formulation for embolization is approximately 20% w/w solution of the macromer irradiated at approximately 30 kGy.

The foregoing detailed description is intended to describe certain examples of the invention only and is not intended to be an exhaustive description of all possible embodiments of the invention. It is top be appreciated that various changes, modifications and alterations may be made to the above-set-forth description without departing from the intended spirit and scope of the invention.

Claim 1 of 29 Claims

What is claimed is:

1. A method for treating a disease, deformation or disorder of a human or veterinary patient, said method comprising the steps of:

(A) providing a quantity of a biocompatible, hydrophilic polymer which biodegrades to form polymer fragments that are of sufficiently low molecular weight to undergo renal clearance without causing substantial irreversible damage to the patient's kidneys;

(B) irradiating the polymer so as to cause crosslinking of the polymer; and,

(C) introducing the crosslinked polymer into or onto the patient's body.
 

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