Title:  Treatment of alopecia

United States Patent:  6,844,326

Issued:  January 18, 2005

Inventors:  Li; Lingna (San Diego, CA)

Assignee:  AntiCancer, Inc. (San Diego, CA)

Appl. No.:  199757

Filed:  July 17, 2002

Abstract

A composition and method for treating and monitoring alopecia by altering hair follicle cells to produce a cell cycle inhibitor is disclosed.

Description of the Invention

TECHNICAL FIELD

This invention relates to methods for specifically delivering cell cycle inhibitors to hair follicles to inhibit alopecia. In particular, the invention relates to specifically delivering the cell-cycle inhibitor, p21 to hair follicles.

BACKGROUND ART

Many chemotherapeutic agents, for example, antimetabolites (methotrexate, 5-fluoouracil, cytarabine), alkylating agents (cyclophosphamide, mechlorethamine, dacarbazine, ifosfamide), antineoplatic antibiotics (bleomycin, actinomycin D, daunomycin, doxorubicin, mitoxantrone), the vinca alkaloids (vincistine, vinblastine) and taxanes (Taxol, Taxotere), produce an anagen effluvium to induce alopecia by killing the active proliferating cells of the hair matrix. Scalp hair is particularly sensitive since 85% of scalp hair is in anagen phase. Chemotherapy-induced alopecia (CIA) is thus a major problem in clinical oncology, which can be a major obstacle for patients to accept chemotherapy. It is particularly devastating to women as alopecia-inducing agents such as the taxanes are becoming more frequently used in breast and ovarian cancers.

Surgical transplantation of small, discrete, skin areas having viable follicles to areas having inactive follicles is expensive, labor-intensive and relatively short-lasting. Also, as described by R. F. Oliver et al. in U.S. Pat. No. 4,919,664, follicular dermal cells can be inserted into a skin incision, resulting in hair growth along the incision. However, this is a complex technique that does nothing to stimulate existing follicles. Treatment of the hair and skin with various creams or lotions with biologically active ingredients to improve hair growth has generally low efficiency. Attempts to follow this approach have been ineffective, possibly because of the inability of stimulators to penetrate the cellular membrane of hair follicle cells and to enter into the cells where their action is needed.

Liposomes, which are artificial phospholipid vesicles, have been successfully used for delivery of different low-molecular-weight water-soluble and oil-soluble compounds into different cells. See, for example, G. Gregoriadis, Trends in Biotechnology (1985) 3:235-241 and K. H. Schmidt, ed., Liposomes as Drug Carriers, Stuttgart:George Thieme Verlag (1986). The applications and issued patents from which priority is claimed describe the use of liposomes to target hair follicles specifically.

No pharmacological agent inhibits CIA in a reliable, cost-efficient, unharmful and long-lasting manner. The treatment of human hair loss, especially CIA is important and beneficial for cancer patients and other persons generally. Thus, new agents and treatments for the prevention of CIA are needed.

It has been verified, as exemplified below, that chemotherapy-induced alopecia can be almost completely prevented in skin histoculture by liposome targeting of the gene encoding the cyclin-dependent-kinase inhibitor p21 to the hair follicle.

SUMMARY OF THE INVENTION

Generally, the present invention is directed to methods to inhibit chemotherapy-induced alopecia by selective delivery of expression systems for cell cycle inhibitors to the hair follicles.

In another aspect, the invention is directed to a method to monitor the expression of p21 in cells which method comprises assessing the fluorescence of the cells that have been treated with an expression system for a fusion protein, which comprises an amino acid sequence conferring p21 activity linked to an amino acid sequence which effects emission of fluorescent light. The fluorescence may be observed in vitro or in vivo, even in the live subject.

DETAILED DESCRIPTION OF THE INVENTION

Cyclin-dependent kinase inhibitor (CDIs) proteins are important because they regulate CDKs at specific points in the cell cycle and can therefore negatively control progression of the cell cycle. See Hirama et al. Blood (1995) 86:841-854; Polyak et al. Gene Dev (1994) 8:9; Toyoshima et al. Cell (1994) 78:67; Serrano et al. Nature (1993) 366:704; Serrano et al. Science (1995) 267:249. The mammalian CDIs include p21, p20, p27, p28, p16, p15 and p18. The p21 protein is a potent inhibitor of all cyclin/CDK complexes tested, including cyclin/CDK2 and cyclin D/CDK4. The p21 protein can inhibit DNA replication in the absence of cyclin/CDK by binding to PCNA and inhibiting its ability to activate DNA polymerase .delta. and thus p21 can stop DNA synthesis and inhibit cells from entering S-phase. Waga et al. Nature (1994) 369:574. Therapeutic application of p21 puts the hair-follicle into a resting state (telogen) to protect against toxic events such as chemotherapy-induced alopecia (CIA).

The hair follicle is a complex mini-organ driven by a biological clock in a rhythmic, cyclic fashion from stages of resting (telogen) to growth (anagen) and via a short regression phase (catagen) back to the telogen phase. See Chase, H. B. Physiol Rev (1954) 34:113-26; Orfanos, C. E., Happle, R. (eds.) Hair Growth and Hair Diseases, Springer, Berlin (1990); Rook, A., Dawber, R. (eds.) Diseases of the Hair and Scalp, Blackwell, Oxford (1992). Cytotoxic agents damage cycling hair matrix cells to cause "anagen effluvium" and eventually induce alopecia. See Hood et al. Cancer Medicine, 4th ed. Williams & Wilkins. 1:3141 (1997).

An in vitro model for studying the anagen phase of the murine hair cycle for almost the entire duration has been developed. In this model, catagen can be induced by chemotherapeutic drugs. This in vitro system is based on the collagen-sponge-gel-matrix supported histoculture technology adapted for hair-producing skin culture. See Li et al. Proc Natl Acad Sci USA (1992) 88:1908-12; Li et al. In vitro Cell Dev Biol (1992) 28A:479-481, 695-698, 679-681; Li et al. In vitro Cell Dev Biol (1993) 29A:192-194, 258-260,449-450; Li et al. In vitro Cell Dev Biol (1994) 30A:135-138. The skin histoculture technology provides an opportunity to observe, characterize, analyze and manipulate the development of anagen (growth) and catagen (breakdown) in mature hair follicles in vitro in full-thickness skin. The skin histoculture system may also be used to induce CIA with actual hair loss observed in vitro.

Recently, two animal models have been used for studying CIA. It has been demonstrated that the new-born rat is a model for CIA with doxorubicin and cyclophosphamide/cytosine arabinoside. See Jimenez et al. Cancer Invest (1992) 10:271-276. The doxorubicin-induced alopecia in new-born rats was prevented by 1,25-dihydroxyvitamin D₃ and the cyclophosphamide-induced alopecia was prevented by ImuVert/N-acetylcysteine. Also reported is a murine model for inducing and manipulating hair follicle regression (catagen) and CIA. See Paus et al. J Invest Derm (1994) 103:143-147; Paus et al. Cancer Research (1996) 56:4438-4443; Paus et al. Am J Pathol (1994) 144:719-734. The massive catagen development in anagen C57B1-6 mice can be achieved after topical treatment with dexamethasone once daily. CIA evidenced by dystrophic anagen, catagen and disruption of melanization of hair follicles was established in C57B1-6 mice with a single intraperitoneal injection of cyclophosphamide. See Hoffman, R. M. J Cell Pharmacol (1991) 2:189-201. Topical application of immunophilin ligands such as cyclosporin A and FK506 can induce active hair growth in telogen C57B16 mice and also inhibit massive, dexamethasone-induced, premature catagen development. See Maurer et al. Am J Pathol (1997) 150:1433-1441.

This invention provides uniquely effective protocols and materials for the treatment of CIA, as well as assay systems for monitoring hair follicle cells and hair growth. The cyclin-dependent kinase (CDK) inhibitors including p21, p16 and p27 may be used to put the hair follicle into a resting, chemo-resistant phase to prevent CIA.

The expression systems employed in the present invention generally comprise a nucleotide sequence encoding a cell-cycle inhibitor such as the p21 protein operably linked to sequences which effect expression of the coding sequence. Vectors for delivery of the nucleotide sequence encoding a cell-cycle inhibitor may also effect the insertion of the nucleotide sequence into the genome of the host, thus employing the endogenous control sequences to effect expression.

If the vector contains an expression system, suitable promoters and enhancers can be used. General constitutive promoters such as SV40 or CMV promoters can be included, along with their enhancer elements, or tissue-specific promoters may be used to enhance specificity. Means to construct suitable vectors for delivery of a gene along with provision for its expression are well known in the art.

In order to effect the modification of cells for the expression of the cell-cycle inhibitor, the expression system or integrating encoding nucleotide sequence must be formulated so as to enter the cell. Integration of the desired nucleotide sequences into viral vectors, such as adenovirus may provide this means of entry. However, retroviral vectors, or other mediators of cellular uptake, such as lipids, or various liposomal type formulations or emulsions are preferred.

It is also part of the invention to employ the protein exhibiting cell-cycle inhibition as a fusion protein to a reporter amino acid sequence, most preferably an amino acid sequence which confers fluorescence on the fusion protein. The use of green fluorescent protein (GFP) to confer fluorescence on a fusion protein is well understood in the art; see, for example, Chalfie, M., et al. Science (1994) 263:802-805. The expression system may be targeted to the hair follicle cells of interest by utilizing liposome-mediated delivery as described in U.S. Pat. No. 5,641,508, filed Jan. 13, 1994, and incorporated herein by reference.

The invention contemplates using the expression system to study and treat chemotherapy-induced alopecia (CIA). Similarly, the invention contemplates using the expression system in in vitro and animal models to determine the effects of various substances on CIA.

A therapeutic composition contains the expression system of the present invention. A therapeutically effective amount of the expression system and, if present, other beneficial compounds, is a predetermined amount calculated to achieve the desired effects, i.e., to effectively affect the pigmentation of the skin or hair cells. Thus, an effective amount can be measured by improvements in one or more symptoms associated skin or hair cell growth in the subject.

The dosage can be adjusted by the individual physician in the event of any complication. The compositions are administered in a manner compatible with the dosage formulation, and in a therapeutically effective amount. The quantity to be administered depends on the subject to be treated, capacity of the subject's system to utilize the active ingredient, and degree of therapeutic effect desired. Precise amounts of active ingredient required to be administered depend on the judgement of the practitioner and are peculiar to each individual. However, suitable dosage ranges for systemic application are disclosed herein and depend on the conditions of administration. Suitable regimes for administration are also variable, but are typified by an initial administration followed by repeated doses at one or more hour intervals by a subsequent administration.

The choice of vector to which a DNA segment of the present invention is operatively linked depends directly, as is well known in the art, on the functional properties desired, e.g., protein expression, and the host cell to be transformed, these being limitations inherent in the art of constructing recombinant DNA molecules. However, a vector contemplated by the present invention is at least capable of directing the replication, and preferably also expression, of the beneficial protein structural gene included in DNA segments to which it is operatively linked.

In preferred embodiments, a vector contemplated by the present invention includes a procaryotic replicon, i.e., a DNA sequence having the ability to direct autonomous replication and maintenance of the recombinant DNA molecule extrachromosomally in a procaryotic host cell, such as a bacterial host cell, transformed therewith. Such replicons are well known in the art. In addition, those embodiments that include a procaryotic replicon also include a gene whose expression confers drug resistance to a bacterial host transformed therewith. Typical bacterial drug resistance genes are those that confer resistance to ampicillin or tetracycline.

Those vectors that include a procaryotic replicon can also include a procaryotic promoter capable of directing the expression (transcription and translation) of the beneficial protein gene in a bacterial host cell, such as E. coli, transformed therewith. A promoter is an expression control element formed by a DNA sequence that permits binding of RNA polymerase and transcription to occur. Promoter sequences compatible with bacterial hosts are typically provided in plasmid vectors containing convenient restriction sites for insertion of a DNA segment of the present invention. Typical of such vector plasmid are pUC8, pUC9, pBR322 and pBR329 available from Biorad Laboratories, (Richmond, Calif.) and pPL and pKK223 available from Pharmacia, Piscataway, N.J.

Expression vectors compatible with eucaryotic cells, preferably those compatible with mammalian cells, and particularly hair follicle cells, can also be used to form the recombinant DNA molecules for use in the present invention. Mammalian cell expression vectors are well known in the art and are available from several commercial sources. Typically, such vectors are provided containing convenient restriction sites for insertion of the desired DNA segment, and provide the signals required for gene expression in a mammalian cell. Typical of such vectors are the pREP series vectors and pEBVhis available from Invitrogen (San Diego, Calif.), the vectors pTDT1 (ATCC #31255), pCP1 (ATCC #37351) and pJ4W (ATCC #37720) available from the American Type Culture Collection (ATCC) and the like mammalian expression vectors.

Particularly preferred are mammalian expression vectors which allow the expression of the gene in a tissue-specific manner, in this case by the action of a regulatory promotor that will limit gene expression to hair follicle cells.

Successful transformation of the target tissue can be confirmed by evaluation of the target tissue for indicia of function exerted by the administered beneficial compound. For example, where the compound is a nucleic acid expressing p21 protein.

Claim 1 of 6 Claims

What is claimed is:

1. A method to inhibit chemotherapy-induced alopecia which method comprises

delivering p21 protein to hair follicles of a mammal, said delivering comprising the step of applying topically to skin areas of a mammal having a plurality of hair follicles, an effective amount of a nucleotide sequence encoding a nucleotide sequence encoding a cyclin-dependent kinase inhibitor p21 protein which upon expression provides p21 protein to the hair follicle cells of said mammal.

____________________________________________
If you want to learn more about this patent, please go directly to the U.S. Patent and Trademark Office Web site to access the full patent.